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By combining the nuclear binding stains Syto 13 and EB, contrasting colours (green and red, respectively) can be observed for differentiation purposes.
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The same could be observed for adipogenic differentiation, which was generally poor, while variations among the donors could be observed.
The distribution of stage of tumor was found to be insignificant (P = 0.11) between age ≥ 45 years and age < 45 years while significant difference (P = 0.01) was observed for cellular differentiation between both groups.
A similar time course of adipogenic differentiation was observed for the two cell types, and oil red O-triglyceride-specific staining was obtained from day 3 of differentiation.
This latter mechanism has been observed for TLR4-induced plasma cell differentiation [50].
On day 7, the strongest induction of monocyte-like differentiation was observed for 1,25(OH)203, 20(OH)D3 and pL with other compounds being less potent, and 7DHP and 20(OH pD having no effect (Fig. 4D, Table S2K).
The highest levels of differentiation are observed for comparisons with SB (Table 3), which is likely due to the inflation of the Kst statistic by the generally low overall level of sequence diversity within this sample [33].
A bias towards oligodendroglial and astroglial differentiation was observed for H3.3 and H3.1 tumours, respectively.
Overt neural differentiation was observed for ESΔN-iNanog and ESΔN-iEsrrb cells in the absence of transgene induction.
A similar consequence of a lack of genetic structuring and low population differentiation was observed for these two possibilities; however, there were slight differences in the outcomes.
A trend of increased adipogenic differentiation was observed for yMSCs cultured in aSerum (1.34±0.20) compared with their counterparts in ySera (1.11±0.07, P=0.112), but did not reach statistical significance.
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