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An increased amount of IGROV1 cells arrested in the G2/M phase of the cell cycle could be observed for cells treated with 10 nM PTX (46.5% ± 3.4%, Figure 1b) compared to untreated control cells (25.2% ± 7.3%, Figure 1a).

Similar, but significantly weaker effects could be observed for cell lines HT1080, DU-145 and SW480 after 48 h treatment with compounds 5 and 6 (data not shown).

The reverse is observed for cells in late S-phase (top row).

After the initial rapid rate of repair, a slower rejoining rate was observed for cells incubated in growth medium.

In general, consistent growth kinetics was observed for cells cultured on the plate.

The same was observed for cells transfected with control GFP (Fig. 2c, right upper panel) or GFP-NP/PR8 (Fig. 2c, right lower panel).

Figure 1B shows that at t = 4 hrs, the percent of BrdU-containing cells in the early S-phase was slightly lower in PREP1 down-regulated than in control cells, whereas the opposite was observed for cells in late-S.

In comparison, no corrosion products are observed for cells containing gel electrolytes, and the Nyquist plots indicate that corrosion does not occur appreciably.

Specific growth rates of 0.047 h−1 and 0.044 h−1 were observed for cells adapted to OptiPRO and VP-SFM respectively, compared to 0.035 h−1 in 5% FBS DMEM.

A four-fold increase in transcript level of Col-1 and a higher level of collagen matrix incorporation were observed for cells grown on denatured collagen versus cells grown on non-denatured collagen.

However, an opposite profile was observed for cells recovered from biofilms grown on the SS for 48 h.