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The data also provide further evidence that the loss of activity of the Δ15 truncation against eEF2 is unlikely to be due to an increase in the Km value for this substrate, since, if that were the case, detectable activity should be observed at increasing substrate concentrations.
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The S10-A10 joint configuration has an acceptable resistance (i.e. about 80%% of un-aged sample) at low ageing time (until 2 weeks); afterward, some broken samples were observed at increasing ageing time.
As for the native enzyme, a decreased inhibitory role of citrate was observed at increasing substrate concentrations for both mutants (Fig. 3D).
A gradual increase in enzyme activity was observed at increasing NaCl concentrations.
This complementation was found to be pH-dependent, with progressively better growth being observed at increasing pH, and was thus indicative of transport of neutral NH3 rather than charged NH4+[ 54].
The zone of inhibition ranged between 17 29 mm at 32 mg mL−1 of chlorogenic acid and a consistent increase in zone sizes were observed at increasing concentrations of the chlorogenic acid.
The predominant influence of steric hindrances on Eu3+ coordination centre was observed at increase of the size of aromatic fragment of carboxylate anions.
Yet, the trend of gradual increase in the proportion of downregulation can be observed at each increase in the number of target sites, and many of these steps are associated with significant p-values.
Subsequently, the ATP hydrolysis rate decreased sharply and a constant relative rate of phosphate production (3.2 ± 0.2 μmof/L·min−1 of Pi) was observed at both increasing and steady states of polymerization.
A good correlation of the two displacement values with the temperature was observed at an increase of nearly one order of magnitude over a temperature increases from 20 K to 40 K (yellow area in Fig. 9).
Similar trends were observed at Kisii (32% increase from January 1987 through December 1999 p=0.0199) and Tabaka (91% increase from January 1980 through December 1999 p<0.0011).
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