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If this assay was employed with test strains with varying growth rates, the luminescence values could be normalized to optical density, although it would need to be noted that the normalized value would be the optical density of the combined cultures and not necessarily reflective of the cell density of the strain being analyzed for AHL production.
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The row data was normalized to optical density of the sample (number of cells) and peak intensity of internal standard (cholesterol).
Log phase cultures of wildtype and mutant cells were normalized to optical densitiy between 0.15 and 0.2 and shifted to fresh medium containing 20 µg/ml nocodazole for 3 hours at 23°C.
Data was normalized to optical density prior to analysis.
All gene expression assays were performed in triplicate, and data was normalized to optical density prior to analysis.
The assays were initiated by spotting 1 µl of an overnight cell culture which has been normalized to an optical density of around 1.5 at OD 600nm onto the agar plates, then incubated at 20°C or 4°C for 3 days or a week, respectively.
In order to minimize the effect of any experimental factor on a spot volume, each spot volume was normalized to the total optical density in each gel image [ 52].
The optical signal was normalized to p/s/cm/sr, and the emission intensity was normalized to the radioactivity injected.
Absolute optical density (OD) was normalized to the ODs of the corresponding bands of β-tubulin loading control and expressed as relative abundance in arbitrary units.
The mean optical density of each spot was normalized to the positive control and compared between control and treated epithelia (Fig. 2C).
In all cases, the optical transmission reported in this paper is normalized to the input power of the simulated structures.
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