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Additional sample sets may be needed for validation of initial studies both in the same populations as well as in other ethnic groups.
Substantial resources will be needed for validation of existing methods, support of novel methods, and enhancement of analytical capabilities.
Once the candidate genes were flagged, more sophisticated statistical methods and re-sequencing of these potential target regions and more would be needed for validation.
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Future studies with a larger sample size followed by functional experiments will be needed for validations.
However, these results should be interpreted with caution given the relatively small sample size, and a larger group in future study will be needed for validations.
Accordingly, the forgoing enrichment of downregulated mitochondrial genes should be noted, however additional bioinformatic tests will be needed for validations.
Future pilot studies are needed for validation and adoption.
Experimental results are needed for validation of unsteady load analyses.
In the case of NDPH, a well designed and controlled pilot study using botulinum toxin A is needed for validation of my finding in this report.
Although more observed data is needed for validation, the relationship model produced in this study can be applied to new data or used for scenario analysis.
Further replication studies are needed for validation.
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