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As deep sequencing projects are completed, additional DNA from study participants will be needed for replication and follow-up studies.
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For example, the Caulobacter crescentus regulator CtrA both transcribes a set of genes involved in replication and binds to the chromosomal origin, silencing initiation of replication, and degradation of CtrA by the Clp proteases is needed for replication to begin [53].
Additional studies are needed for replication of these findings in other populations.
Thus, the data should at this point be cautiously interpreted and new studies are needed for replication of the results.
Further observational and experimental studies are needed for replication and to explore the direction and underlying mechanism of these associations.
In Drosophila, a six-protein origin recognition complex (ORC) recognizes origins of replication, and binding of this complex is needed for replication to begin.
Future studies with more rigorous measurement of infection are needed for replication of these results and to understand potential mechanisms of the observed link.
In a previous study, the DSP activity of VP2 was shown to be needed for virus replication in the cell [ 28].
Even though the genome replication relies on an error-prone RNA-dependent RNA-polymerasis, CHIKV strains S27 and 05.115 Reunion showed in E1 and E2 a low amino acid variation of 0.68% and 3.3%, respectively, and such a low variability may be needed for an effective replication in two phylogenetically distant hosts [ 116, 122].
The extra MCM proteins do however seem to be needed for the recovery of replication in Xenopus extracts in vitro in the presence of inhibitors of ATR [25].
This strain differs by 11 amino acids from the wild type strain ZH548, making it likely that cellular factors required for MP12 replication will also be needed for wild type strains [21].
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