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They also suggested that lengthening the 5′ end of gRNA reduces on-target editing efficiency, as 5′ nucleotides might not be necessary for full gRNA activity.
FT protein interacts with the bZIP transcription factor FLOWERING LOCUS D (FD), which is only expressed in the shoot apical meristem (SAM), and this interaction seems to be necessary for full activation of FT floral promotion [1, 76].
A number of issues will be on the table: exchanging ambassadors; lifting restrictions on Cuban and U.S. diplomatic personnel in each other's country; fugitives and convicts on both sides of the 90-mile-wide Florida straits; property disputes; and financial and technological deals that will be necessary for full business interaction.
GAL2 is a plasma membrane galactose permease that is defective in S288C and might be necessary for full activation of galactose-inducible promoters [31].
Nonetheless, even though the absence of Fur or a decrease in the iron concentration resulted in a clear reduction in hilD expression (Figure 5), both HilD and Fur appear to be necessary for full in vivo expression of the gene.
It is thought to play a structural role in organizing the catalytic region [21], but in certain kinases, phosphorylation of this site was shown to be necessary for full kinase activity [22].
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Those who do mistakenly believe that being a human being is necessary for full moral status might simply correct their mistaken belief when confronted with an actual example of a non-human or borderline-human that was clearly a person.
Access to information systems over the Web is necessary for full participation in modern society.
An amiloride concentration of 10 4 mol/L was necessary for full inhibition of amiloride-sensitive sodium ion (Na+) transport.
Most notably Tesla has chosen not to include Lidar laser-based sensors, a technology most other car makers believe is necessary for full autonomy.
Upstream elements of the DDR cascade are necessary for full blown SASP, and additional crosstalk occurs between the DDR and cytokine secretion.
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be necessary for temporary
be important for full
be necessary for much
be necessary for certain
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