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A given promoter could be mutated at various positions to provide information on its function.
For example, in Noonan syndrome, NRAS or KRAS can be mutated at various positions along their coding sequences in the germline.
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iii) Mutation: The new created individual will be mutated at a definite point in the chromosome. .
In microsatellite unstable tumours MBD4 can itself be mutated at an exonic polynucleotide tract.
(A ) Locations of the mutations in the three genes that were mutated at least three times.
While Trp53, Braf, and Pik3ca are not mutated, Kras is mutated at G12D.
Of the mutated K-ras genes in these patients, 92% were mutated at codon 12, 8% at codon 13, and none of them was mutated at both codons 12 and 13.
Two clones were mutated at position 3 (Y3L and Y3H), while two others were mutated at independent, but neighboring locations (W109R and Y111H).
The disease is very complex genetically, however, and many more genes are mutated at low frequencies.
We found three genes that were mutated at least three times: BEM3 (10/10), NRP1 (5/10), and BEM2 (3/10).
10.7554/eLife.00825.012 Figure 4. MYBL2 is mutated at a low frequency in MDS.
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