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Hematopoietic and muscle stem cells can be more efficiently reprogrammed than differentiated blood or muscle cells, yet similar findings have not been shown in other primary organ systems.
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To test our hypothesis that stem cells are more efficiently reprogrammed into iPS cells than mature ones, we needed to compare cells from the same cell lineage but from distinct developmental stages.
Collectively, these results demonstrate that p53−/− MEFs are more efficiently reprogrammed to hematopoietic progenitors with erythroid, myeloid, megakaryocyte, and B and T lymphoid lineage potential than wild-type MEFs, indicating that P53 expression is a barrier for reprogramming to blood.
Donor cells that are difficult to reprogram can be more efficiently induced into the pluripotent state by adding hTERT and SV40 large T antigen to the reprogramming factors originally used by Yamanaka's laboratory (Park et al., 2008).
Our goal is to be more efficiently productive.'".
Classified ads are more efficiently delivered by websites.
Labour's voters are more efficiently distributed than Conservative voters.
R-cdA is more efficiently repaired than S-cdA.
Buffering genes are more efficiently translated than non-buffering genes.
Although the mechanisms underlying differences in reprogramming efficiency are not yet clear, some cell types might be more easily reprogrammed using specific exogenous factors than others.
This is because this change is seen in the regulatory regions of these genes and is more pronounced in DmES nuclei that are efficiently reprogrammed than in 10T1/2 nuclei.
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