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In general, these probes are synthetic DNA or RNA molecules (with a sequence designed for a specific target molecule) containing a reporter group that can be monitored using fluorescence spectroscopy.
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In order to determine the infection efficiency, green fluorescent protein (GFP) was monitored using fluorescence microscopy after 10 days from infection.
Formation and retention of the depot was monitored using fluorescence molecular tomography imaging.
Separation is achieved on a Alltima 3C18 column using isocratic elution; the drugs are monitored using fluorescence detection.
The hybridization of oligonucleotide sequences complementary to the genes of Shiga toxins (verotoxins) types 1 and 2 of enterohaemorrhagic Escherichia coli (EHEC) and human hepatitis C virus (HCV) was monitored using fluorescence polarization under the reaction condition of high salt concentration (0.8 M NaCl), which was optimized to obtain a higher rate of hybridization.
Probes were co-injected via tail vein and overall fluorescence was monitored using fluorescence tomography (FMT1, VisEn Medical) at various time points (Fig. 2A).
Nanovesicle uptake was monitored using fluorescence microscopy.
Changes in mitochondrial morphology in EYFP- B2-transfected cells were monitored using fluorescence microscopy.
SDS-induced unfolding was monitored using fluorescence spectroscopy as described previously.
At 5 days post infection, embryos were monitored using fluorescence microscopy (Leica MZ16FA).
Distribution of ACF in live parasites was monitored using fluorescence microscopy.
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