Direct access to the microspores speeds up the optimization of culture conditions, and the entire process of microspore embryogenesis can easily be monitored under an inverted microscope (Polsoni et al. 1988; Graner 1996).
Vaz expressed concern that the fugitive, who was being monitored under a terrorism prevention and investigation measures (Tpim) order, may have another passport that he could have used to flee the country.
An ASHP operating in a TBZ was tested in a climate chamber where the performance was monitored under a variety of climatic conditions.
CD measurements were conducted using a JASCO-725 circular dichroism spectropolarimeter equipped with PTC-348 Peltier type single cell holder, and the change in ellipticity at 220 nm was monitored under a constant heating rate (1°C/min) at temperatures ranging from 40 to 105°C.
After the emission of gametes, fecundation was monitored under a microscope; then, after dilution, the larvae were transferred to Iwaki microplates (final volume 3 mL, number 300 larvae/well) and placed in culture with sand extracts at 24 ± 1°C for 24 h.
The presence/absence of the GFP signal has been monitored under a fluorescent microscope from 24 to 48 hpf.
Injections were monitored under a dissection microscope and repeated until the dye filled up the space in the midbrain and cerebellum where projections were observed from the caudal hindbrain (Fig. S1F G).
Epithelial outgrowth was monitored under a microscope at 24 hour intervals, and DMEM growth medium was supplemented with 100 µl fresh medium after 24 hours initially, and subsequently every 48 hours.
Colour development was monitored under a microscope.
