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Pharmacological agents having modulator effect in oxidative stress processes can be monitored at cellular level.
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This cell line constitutes a good model system for studying IGC, because the reversion of an Ig frameshift mutation is AID-dependent and can be monitored at the cellular level by flow cytometry of surface Ig positive cells [34], [35].
In addition, our approach offers the advantage of easily identifying infected cells with GFP expression driven off the bicistronic message, allowing for phenotypic changes in the epithelium to be monitored at a cellular level.
During the follow-up period, plasma viremia was monitored at least every four months, and cellular HIV-1 load was quantified at multiple time points (Table S1).
Another batch of cells exposed to similar conditions was monitored at different time points for its cellular uptake, distribution and clearance for up to 120 h by fluorescent microscopy.
was monitored at 265 nm.
Effluent was monitored at 205 nm.
Fluorescent emission was monitored at 510 nm.
Heme was monitored at 405 nm.
The sample was monitored at 257 nm.
The fractions were monitored at 210 nm.
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