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We demonstrate a method of making such devices much more flexible in that the near-source response can be modified while maintaining gradiometric order.
Guided by the structure of the Cas9 sgRNA complex, we identify regions of sgRNA that can be modified while maintaining or enhancing genome-editing activity, and we develop an optimal set of chemical modifications for in vivo applications.
A modest fraction (approximately 20%) of primary amines of PAMAM can be modified while maintaining the siRNA delivery efficiency of unmodified PAMAM, but higher degrees of amine neutralization reduced the gene silencing efficiency of PAMAM/siRNA delivery vectors.
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The resulting configuration is then used in a rating routine where only the heat transfer surface type on one exchanger side is modified while maintaining all design parameters constant.
By increasing the atom transfer radical polymerization (ATRP) reaction time, the thickness of PMEDSAH was increased and its internal hydrogel architecture was modified, while maintaining its overall chemical structure.
Here we show that chicken PGCs can be isolated, cultured and genetically modified while maintaining their commitment to the germ line.
This initial SAR suggests pharmacophoric elements that may be modified to modulate/increase opioid affinity, while maintaining high affinity for the NOP receptor, to result in potent bifunctional small-molecule NOP/MOP ligands.
Positive end-expiratory pressure, inspiratory oxygen fraction, minute ventilation, and duty cycle remained constant throughout the study, while respiratory rate was modified to maintain constant minute ventilation.
Each of the modules, both SV-callers and pre- and post-processing steps, can be modified by changing the predefined running parameters while maintaining the structure.
The pH condition of the media was modified or maintained by adding either HCl or NaOH.
In different assessments in which the items have varying time demands, the 10-second ceiling may need to be modified to identify rapid-guessing behavior in an effective manner while maintaining the accurate meaning of rapid guessing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com