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Comparing the five successful designs to the unsuccessful cases, we find a trade-off between protein stability and the ability to be modified by phosphorylation.
Some compounds may have multiple states, for example, a protein may be modified by phosphorylation.
Sp1 was shown to be modified by phosphorylation of serine and threonine residues [67].
Although mammalian Cdc6 is known to be modified by phosphorylation and ubiquitinylation [35], [36], and at least two distinct protein bands have been shown to exist for mammalian Cdc45 [37], we cannot be certain that all bands recognized are indeed Cdc6 and Cdc45.
Inositol is a glucose isomer that can be modified by phosphorylation to serve as a second messenger in cells.
GATA2 may be modified by phosphorylation, acetylation and sumoylation and is rapidly turned over by ubiquitination (Towatari et al, 1995; Hayakawa et al, 2004; Minegishi et al, 2005).
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In two designs, Erbin's peptide binding function is modified by phosphorylation, where the presence of the phosphate group destabilizes peptide binding.
Treating the cell lysate with λ phosphatase elimnated the multiple upper bands, suggesting that Atg31 is modified by phosphorylation (Fig. 1B).
An autoradiograph of the purified protein, separated into polypeptides by denaturing gel electrophoresis, indicated that the largest subunit (RPB1) is modified by phosphorylation (Figure 2A).
It has been shown previously that T. brucei RNAP-II is modified by phosphorylation, despite the lack of consensus heptapeptide repeat sequences [20].
Although the RETN expression is under ultimate transcriptional control of CEBPE [12], the activity of CEBPE is modified by phosphorylation [36], heterodimerization with abundant associate proteins [37] and a synergistic effect of MYB and ATF4 [12].
More suggestions(17)
be preceded by phosphorylation
be monitored by phosphorylation
be controlled by phosphorylation
be modified by substitution
be 'rescued by phosphorylation
be promoted by phosphorylation
be modified by introduction
be modified by chemical
be modified by equipment
be inhibited by phosphorylation
be regulated by phosphorylation
be mediated by phosphorylation
be activated by phosphorylation
be affected by phosphorylation
be generated by phosphorylation
be upregulated by phosphorylation
be modified by learning
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