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In addition, AT data could not be modeled because of the limited number of time points.
Regions, which could not be modeled because of the lack of the appropriate template structure, were added "de novo" using the fragment insertion method ROSETTA [ 37].
In the crystal structures of both wild-type PBP2 and PBP2-6140CT (whicontainsins the four C-terminal substitutions but lacks the Asp345a insertion), 10 11 residues of the β3−β4 loop could not be modeled because of flexibility.
The channels between these voids are too narrow to be modeled because of the limitations of this technique, but they are represented by the distorted peak at 6 Å.
The variable N-terminus, which could not be modeled because of the strong divergence and the lack of appropriate template structures, was added "de novo" using the fragment insertion method ROSETTA [ 37].
The structure was determined with two molecules in the asymmetric unit and then refined by alternating rounds of REFMAC refinement and manual building using O. Residues 542 545 of molecule A and residues 504 511 and 543 545 of molecule B could be not be modeled because of weak or absent electron density.
Similar(54)
Because rodent model systems that employ newborn animals to produce lethal infection are generally considered to be farther removed from the human disease being modeled [3], and because of the difficulty of working with newborn mice, we developed a new TCRV mouse infection model based on i.p. challenge of AG129 type I and type II IFN receptor-deficient mice.
Cellulose was modeled as group of microfibrils made of bundles of elementary fibrils.
A non-linear binding activity event is modeled via slight modification of minterm on Eq. (3).
The missing residues (1 25, 46, 47, 58 62, 244 249, and 399 414) are not modeled because of weak or unclear electron density.
Each of them is modeled as follows.
Related(14)
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