As a proof of this concept, we identified giant viruses that were missed in sequence data sets, including among sequences published in Nature in 2010 (Chapman et al. 2010).
It appears that this region was missed in sequencing.
Here too, however, it must be emphasized that the complexity of the repetitive region might have been missed in sequencing assembly.
However, if more than two hematocrit values were missing in sequence and the hemoglobin level was known, hematocrit values were derived by linear regression between all known hematocrit and hemoglobin values within the patient.
It had been observed previously that due to profile drifting, few related sequences may be missed in searches in SCOP-NrichD database and therefore searches were also made in natural sequence database (SCOP-DB) [ 23] and the all hits were pooled together for further analysis.
To address this issue we introduce SAM, a string-based search algorithm that converts query and database sequences to position-free nucleotide strings and thus eliminates the possibility that identical sequences will be missed in a database query.
The lack of nitrate and nitrite reductases is a common feature in all currently available Flavobacteria class genomes, while inorganic sulfur assimilation pathways appear to be missing in two sequenced flavobacteria isolates, Flavobacterium psychrophilum JIP02/86 and the isolate BAL38.
Because the de novo strategy does not require a reference genome for contig assembly, it would still generate contigs in regions where genomic scaffolds may be missing in poorly sequenced or partially assembled genomes.
Many low frequency SNPs that were identified through sequencing of individual DNA samples were missed in the sequencing of pooled templates.
A shotgun sequencing strategy will truncate at the linear sequence level without respect for the conserved base-paired structure, and residues involved in base pairs may be missing in the observed sequence, as illustrated in Figure 1.
