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Because the methylation sites represented in the arrays used for this study were biased towards sites known to be methylated in some cancers, this finding may be consequence of the fact that cell lines represent a 'pure' culture of cancerous cells whereas the primary tumors inevitably contained a mixture of tumor cells and other cells.
The central dinucleotide of the canonical CRE motif, CpG, is known to be methylated in some cases [ 14].
Canonical CREs in CpG islands can still be methylated, in some cases in a tissue dependent or temporal manner.
In the case of HPV16, LCR has been observed to be methylated in some primary cervical carcinomas, especially at E2-binding sites (E2BS; Bhattacharjee and Sengupta 2006; Brandsma et al. 2009; Fernandez et al. 2009).
p16INK4, which is controlling cell cycle progression through the G1 phase, is capable to induce senescence and has been reported to be methylated in some ALCL tumor cells [19].
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It has been previously shown that ∼20% of genes are methylated in A. thaliana diploids.
When a gene is methylated in some but not all normal tissues, the methylation is probably involved in the specification of a tissue type during development [12].
In plants from the RS location, 32.1% of all MSAP fragments were methylated in some samples, while only 14.6% of loci from SM plants were methylated in some samples.
All 67 methylated loci were found methylated in some samples of RS population (32.1% of all loci) but only 30 of these loci were methylated in some samples of SM population (14.6% of all loci).
We demonstrate, through sodium bisulphite sequencing, that the sod2 locus is methylated in some pancreatic cell lines leading to a corresponding decrease in SOD2 expression.
More recently, we have found that the sod2 promoter is methylated in some multiple myeloma cells and diminished expression can be reversed by the methyltransferase inhibitor zebularine (Hodge et al, 2005a), suggesting that repression of SOD2 may occur at the level of epigenetic regulation.
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