The Luciferase activity of each construct was measured using five replicates (independent transfections).
After the third time point, the fluorescence emission of all time points was measured using three replicates.
The transcriptome of a B. cenocepacia population resulting directly from the pathogen-host interaction in sputum from three CF individuals was measured using multiple biological replicates.
The basis of our comparison was a DNA microarray array experiment in which the gene expression in two different haploid strains of S. cerevisiae, RM11-1a and BY4716, in two different growth media was measured using six biological replicates (GEO accession number GSE9376) [ 11].
Iron and sulfur concentrations were measured using ICP-AES, with replicate measurements of Fe by flame and graphite furnace AA.
Skin colour of 20 fruit from each replicate was measured using a colorimeter (Konica Minolta, CR-400, Japan) with a 6-mm aperture size, which provided L* and a* values according to the system established by the Commission Internationale de L'Eclairage (CIE, International Commission on Illumination).
Reproducibility of the FF and FFPE replicates was measured using Pearson correlation, whereas comparison between the FF and FFPE samples was done using a Z-score analysis.
To quantify the fluorescence signal, the mean intensity of the respective single channel from 2-4 frames of three biological replicates was measured using ImageJ (NIH) and mean value and standard deviation were calculated using the Origin software package (Originlab).
Leaf chlorophyll (chl) content was measured using three fully expanded leaves in each replicate with a non-destructive dual-wavelength chl meter (SPAD-502, Minolta Corp., Ramsey, NJ, USA).
Activation of Z-DEVD-R110 by the activity of caspases 3 and 7 allows the R110 group to become intensely fluorescent (Exitation499 nm/Emission521 nm), which was measured using the Synergy4 microplate reader in four replicates.
