Microfracture surface area was measured using classical stereological techniques outlined by Underwood (1969) and further described by Wu et al. (2000) and Heap et al. (2014).
Screening capabilities of HRM and the consistency of the analysis were measure using classical metrics, such as sensitivity, specificity, false negative and positive rates, considering sequencing analysis as the standard measurement for both frozen and paraffin-embedded extracted samples.
Slow release diffusion coefficients (D) were measured using the classical diffusion equation and found to be around 8×10−5 cm2/s.
At a given position, amino acid conservation is inversely related to amino acid diversity, which in turn is measured using the classical, base-2 logarithmic definition of information content in "bits" [81].
Neophobia, defined as the aversion to approach novel objects [5], was measured using the classical approach of placing an unfamiliar object next to the animal's usual feeding spot [7].
Platelet aggregation was measured using a 96-well plate modification in classical light transmission aggregometry developed in our laboratory 11.
Platelet aggregation in response to incremental concentrations of five agonists was measured using a 96-well plate modification in classical light transmission aggregometry, as previously described 11.
Agonist-induced platelet aggregation in patients with metastatic cancer and healthy controls was measured using a 96-well platelet modification in classical light transmission aggregometry.
The ERB of the auditory filter is assumed to be closely related to the critical bandwidth, but it is measured using the notched-noise method [27,28,31,22,5] rather than on classical masking experiments involving a narrowband masker and probe tone [41,42,39].
