Flagellum length was measured either using the MAb25 axoneme marker or from phase contrast images of detergent-extracted cytoskeletons.
Chemotaxis of murine thymocytes was measured either using a 48-well transwell chemotaxis chamber, with 5 µm pore-sized polyvinylpyrrolidone-free filters (NeuroProbe) or using polycarbonate transwell inserts (see below).
The response functions of the activators were measured either using the Dox-inducible pTRE-tight promoter, as above, or the DAPG-inducible pPhlFR promoter from this study.
Transfection efficiency was measured either using a Guava EasyCyte mini flow cytometer (Guava Technologies, Hayward, CA, USA) or a FC500 flow cytometer (Beckman Coulter, Krefeld, Germany) usually 48 h after transfection.
This can be measured either directly using short axis images through the tricuspid valve or indirectly from RV volumes of the short axis and forward flow obtained from the pulmonary artery.
This can be measured either directly, using an isotopic technique based around the permeability of H2O into the matrix and the exclusion of [C]-sucrose, or by light scattering (see [165,182]).
(Table 1) Time spent by the providers and associated activities will be measured either as resources/inputs used or the time spent by the health care personnel.
Until an incidence assay that is not impacted by ARV use is available, current incidence assays should only be applied to settings where ARV use can be measured, either on the basis of survey participants' self-report [30] or by using laboratory methods to test for the presence of ARV markers in the blood.
One example is dyspnoea relief which could be measured using either a Likert scale where the patient compares their symptoms to baseline or a visual analogue scale where the patient rates their symptoms at each point in time and effects could be measured either at 6 and 24 h or over 5 days.
H2O2 was measured either by using the Amplex Red assay or by its conversion to hydroxyl radicals in the presence of iron using the terephthalate assay.
