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HRV can be measured by continuous electrocardiography over a 24-h period, but shorter recordings can also be utilized.
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Heart rate (HR) and blood pressure (BP) were measured by continuous measurement of finger BP using a Finapres (Ohmeda Finapres type 2300E, Blood Pressure Monitor) and the software Vsrrp98 [ 45].
Weight change kinetics were measured by continuous thermogravimetry.
Cu2+-induced oxidation of LDL was measured by continuous monitoring of conjugated dienes.
Release of ρ-nitrophenol (ρNP) was measured by continuous monitoring at 401 nm.
The ammonia content of the aqueous supernatant was measured by continuous flow analysis using a Series 2000 Analyzer.
The sulfur isotopic composition of the resulting sulfur dioxide (SO2) was measured by continuous flow GS-IRMS (Thermo, Delta V Plus).
The nitrate content of the aqueous supernatant was measured by continuous flow analysis using a Series 2000 Analyzer (Burkard Scientific, Uxbridge, UK).
More importantly, kinetics of the G T, G A and G G mispair formation and extension under single turnover conditions were measured by continuous fluorescence-based assay for the first time.
Total %C and δ13C values were measured by continuous flow isotopic ratio mass spectrometry as described elsewhere, [13 15] Note that %C and δ13C values refer to total soil carbon, including organic and inorganic (carbonates) carbon.
Barium sulfate for δ18OSO4 was pyrolyzed at 1,450°C in a Temperature Conversion Element Analyzer (TC/EA), and the resulting carbon monoxide (CO) was measured by continuous flow GS-IRMS (Thermo, Delta V Plus).
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