When the lithium carbonate-treated males were mated with normal cyclic females, the fertility index declined to 50% even after 30 days of withdrawal of lithium carbonate treatment.
Ten weaning female rats (VAN group) were fed with a diet which contents 4 IU vitamin A/g diet, while 20 rats (VAD group) were maintained on a diet without vitamin A. After 10 weeks of feeding, all the female rats were mated with normal male rats.
For further analysis of germline transmission, 4 live chimeras derived from Ws-4-2 were mated with normal males or females.
Immunized and control female fish were mated with normal male D. rereio at a female to male ratio of 2∶1 prior to the primary immunization and every 7 days post the primary immunization (zebrafish reproduce eggs weekly in appropriate conditions).
The male rat at 48 h after treatment with siRNA was mated with normal female rats; they were maintained together overnight, vaginal smears of the females were taken in the following morning and examined microscopy; the mated male rat was sampled for determining the knockdown efficiency.
The diabetic female mice were mated with normal male mice and examined daily for the presence of copulation plugs.
The hTNF-expressing transgenic mice (Tg197) and their normal littermates were mated with the H-2Kb-tsA58 SV40-TAg (simian virus 40 large tumor antigen) transgenic mice [ 24].
For each experiment, two normal female mice were mated with one Pten+/+ or Pten−/− male for 2 weeks and then embryos were counted.
In a breeding colony, phenotypically normal heterozygous (N) females were mated with homozygous dwarf (df/df) mutant males to produce the Ames dwarf and N mice.
Seven male homozygotes were mated with wild-type females and each sired litters of normal size.
