The reads that did not align to the genome but were mapped to potential splice junctions by TopHat were considered to bridge splice junctions.
The functional modularity from GenRev can be mapped to DAVID to annotate the potential functions involving T cell and lymphocyte activation, cell mediated cytotoxicity, DNA and calcium binding and inflammatory response.
The reads that did not align to the genome but that were mapped to these potential junctions by TopHat were considered to bridge splice junctions.
Both overexpressed and underexpressed candidate genes of ZGP vs ZBNP groups were mapped to their potential molecular, cellular and biological functions using the Ingenuity Pathways Analysis (IPA) software [ 58].
For single-molecule maps that were partially aligned to the EBV reference maps, unmapped portions that were >100 kb and had a minimum of 10 nicks were mapped to hg38 to determine potential EBV integration sites.
Illumina sequences (35 nucleotides) were mapped to the plasmid to identify potential contaminants using CLCBio Genomics workbench software package.
The observed phenotypes have not been mapped to specific transcripts, and the potential functional involvement of these microRNAs remains unclear.
After that, the remaining unannotated reads were mapped to miRBase 20.0 to predict potential miRNA, a total of 3383 and 19743 predict tags mapped in control and infected library, respectively.
In fact, in human mature gait, the temporal activation and deactivation of the involved cortical and subcortical (such as the central pattern generators) structures, as well as the chronological sequence of the movement-related potential, were mapped to specific phases of the gait leg movements [ 37].
A second interesting implication pertains specifically to the transcripts with high protein-coding potential that were mapped to PSR.
