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Additionally, if there are multiple variants under a read's mapping, the read may be mapped to multiple positions in the genome, but usually only the best mappings are reported.
As a result, an ORF may also be mapped to multiple samples.
We found 787 sequences (2.6%) only that can be mapped to multiple locations in the genome, which suggests a relatively low level of gene duplication events.
In this mapping analysis, when a read could be mapped to multiple positions on the reference sequence, the one where the number of mismatches was the smallest was chosen; if there were multiple candidates for the least mismatch, the read was mapped to every position as if it was multiplied.
It is critical as multiple reads can be mapped to multiple locations in the genome.
Reads that could be mapped to multiple positions in one or both genomes were discarded.
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Finally, we examined the reads that were mapped to multiple locations (multi-mapped reads; Additional file 1: Figure S1, step 5).
Third, five commonly used gestures are designed to demonstrate the way a gesture is mapped to multiple semantics.
Of the 6 to 30 million quality-evaluated reads (Passed Filtered reads) from each library, 12.3% to 39.8% were mapped to single locations (unique) in the genome whereas 23.7% to 45.7% were mapped to multiple locations in the genome.
In this analysis, the reads that were mapped to multiple positions on the genome were excluded.
Oligo sequences were mapped to multiple databases, including RefSeq, UniGene, Ensembl, TIGR, and genomic coordinates to maximally determine gene identity and function.
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