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First, out of 11,542 SNPs in the original dataset, 105 SNPs were removed because they could not be mapped to human genome with NCBI build 36.
Using Bowtie 2 aligner, about 647 million reads (~97%) can be mapped to human cDNAs.
One sample (run accession ERR009139) is excluded due to an abnormally low rate of reads that can be mapped to human genome (~19%).
In addition, the proportion of tammar contigs mapped across species varies from 23% in zebrafish or 35% in chicken to 59% with the marsupial opossum, while 49% can be mapped to human mRNA sequences and 38% in the platypus.
For all analyses, the 11,131 probesets on the Ambystoma GeneChip that could be mapped to human orthologs in the Panther database were used to generate expected values (i.e., as the background).
Understanding which cell lines best represent the epithelial vs the mesenchymal phenotype will help determine the best models for subsequent drug intervention and target validation studies and will allow cell lines to be mapped to human tumors.
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These mouse genes were mapped to human based on the Human and Mouse Orthology in the MGI database to obtain the orthologous human genes.
The gene was mapped to human chromosome 4q26 q27.
The gene is highly conserved in evolution and has been mapped to human chromosome 21.
The gene had four exons separated by three introns and was mapped to human chromosome 12q13.
Probes were mapped to human chromosomes according to NCBIv35 (hgenomeenome assembly.
More suggestions(16)
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