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The collected stool will be transported to an off-site sorting facility, A. lumbricoides and hookworm will be manually separated from the stool, and the number and sex or worms recorded for each individual.
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The skins were manually separated from pulp, weighed and frozen at −20°C until analyzed.
Briefly, the amnion was manually separated from the chorion, washed extensively in phosphate-buffered saline (PBS) containing 100 U/ml penicillin and 100 g/ml streptomycin, and cut into small pieces.
Fibers from 28 and 40 DPA were manually separated from the seed.
To maintain pluripotency, colonies were manually separated from feeder cells and morphologically identifiable differentiated colonies.
Oocytes were manually separated from the granulosa cells upon hyaluronidase digestion, to discriminate populations.
While frozen the berries were manually separated from stems, leaves, insects, and other debris.
Then, leaves were manually separated from the stems, dried, and ground finely to obtain A. annua leaf powder.
Each fibre was manually separated from both plant types and measured in a light microscope at 10x magnification and diameter was calculated.
The seeds were manually separated from the berries and extracted with 125 mL of the buffer solution for 144 hours at 30°C.
Eggs were manually separated from the adults, rinsed free of faeces and debris, and raised at 28.5°C in embryo media (480 mg Instant Ocean salts, 16 mL 100% v/v methylene blue, 8 L system water, [ 75]) until they reached the desired stage.
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CEO of Professional Science Editing for Scientists @ prosciediting.com