Experimental studies of CWD are costly and challenging to conduct because they require large numbers of animals to be maintained under controlled conditions for long periods of time, given the long incubation period for CWD.
Another set of trays was maintained under control (non-flooded) conditions in an adjacent area.
Three-leaf stage plants were maintained under control conditions or exposed to 4 °C for the specified periods of time.
Analysis of protein extracted from glucocorticoid-deprived cells that had been maintained under control conditions or exposed to wortmannin/PI-103 showed that both substances reduced (∼70%) the abundance of Thr346/356/366-phosphorylated NDRG1, with no effect upon the overall levels of NDRG1.
Rats were maintained under controlled temperature of 24 ± 2°C and relative humidity of 60% ± 10% with a 12-h light/dark cycle.
C2C12 cells were maintained under controlled CO2 atmosphere and temperature.
Plates were maintained under controlled laboratory conditions.
The rats were maintained under controlled conditions with food and water being offered ad libitum.
Mice were maintained under controlled temperature, humidity, and 12 hour light/dark cycles.
Each cell culture was maintained under controlled conditions in stages lasting a month for both cases.
The parental cultures were maintained under controlled conditions in incubators and supplied regularly with dung and sugar water.
