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Moreover, parasitic diseases can be maintained indefinitely in human populations that are without access to medical relief to break the parasite infection cycle.
Using leftover frozen embryos from in-vitro fertilization clinics in Wisconsin and Israel, Thomson and colleagues isolated stem cells and have shown that they can be maintained indefinitely in the lab -- can be grown, frozen and then thawed, and still retain their power to develop into, say, heart-muscle cells or brain cells.
These cells can be maintained indefinitely in culture and yet maintain competence to produce all the cells within a fetus.
Importantly all of these human pluripotent stem cells, with the exception of embryonal carcinoma cells, can be maintained indefinitely in the laboratory with normal karyotype to provide a scalable source of normal (or diseased) human cells for research and clinical applications.
This cell line can be maintained indefinitely in culture under permissive conditions but when cultured under non-permissive conditions, large T antigen expression is reduced substantially, leading to increased p53 activity and reduced cellular proliferation.
Mouse embryonic stem (ES) cells, derived from the inner cell mass (ICM) of blastocysts [1], [2], can be maintained indefinitely in vitro [3] in an undifferentiated self-renewing state in the presence of Leukemia Inhibitory Factor (LIF) [4], [5] and Bone Morphogenetic Proteins (BMPs) [6].
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Once established in humans, the diseases could be maintained indefinitely, if the numbers of susceptible individuals remained above a certain threshold and in frequent contact with diseased persons.
A plasma in stable equilibrium can be maintained indefinitely if the leakage of energy from the plasma is balanced by energy input.
For analysis of chronic TXNIP protein expression cells were maintained indefinitely in DMEM with 5 or 20 mM glucose.
Indeed, in a technological breakthrough, these HAT-induced de novo CENP-A arrays can even lead to the formation of stable HACs that are maintained indefinitely in human cell lines that have previously proven refractory to HAC formation.
In the stochastic regime, LD oscillations can be maintained indefinitely even in the alternative model.
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