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Given that different compounds have varying chemical, physical and biological properties (e.g. small molecules versus antibody versus RNA molecule), the stability and formulation must be maintained for maximum efficacy.
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Responses obtained should be maintained for the maximum time period, which will require the development and implementation of novel antimyeloma therapies suitable for the maintenance setting.
Whereas maximum cell viability could be maintained for 3 days, it dropped to 30%% after 9 days in batch culture for both cell clones.
Aftereffects were maintained for a maximum of 9.8 and 6.6 min after the 1-Hz and cTBS protocols, respectively.
The systems were maintained for a maximum of 228 h.
The cell lines were maintained for a maximum of 20 passages to minimize any long-term culturing effects.
Following the first three eculizumab administrations, complete TCB was maintained for a maximum of 4 days.
The incubation time after the heat shock treatment during transformation was maintained for a maximum of 40 min to avoid repetition of similar types of clones in the library.
After retroviral infection and primary puromycin selection at 3 μg/ml for MDA-MB-231 and 10 μg/ml for MDA-MB-453, cell pools of MDA-MB-231/mir-4728, MDA-MB-231/Vector, MDA-MB-453/mir-4728, and MDA-MB-453/Vector were maintained for a maximum of 10 culture passages under lower puromycin concentration (1 μg/ml).
In three patients, therapy with azathioprine was maintained for more than the maximum latency period of six months (268, 235 and 673 days) and interrupted later in the disease course.
The treatment was maintained for another 4 weeks at the maximum tolerated dose.
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