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Spartina alterniflora has specific nitrogen, phosphorous, sulfur, magnesium, and iron requirements (Broome et al., 1975) that can be maintained by replacing synthetic seawater solutions at regular intervals or adding nutritional supplements such as diluted Hoagland's solution (Merino et al., 2010).
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The commercial role of the canal at that time was to provide water to industries near Swansea, so the water flow had been maintained by replacing the missing arch with pipes.
This culture was maintained by replacing the culture medium and assaying for sub-genomic DENV RNA every seven days.
Sink conditions were maintained by replacing an equal volume of release medium each time.
Cultures were maintained by replacing 50% of the medium on days 4, 7, 10 and 12.
Drug release was monitored for 12 h and samples were withdrawn periodically and sink conditions were maintained by replacing with equal amount of fresh dissolution medium.
Oxygen-glucose deprivation (OGD) was achieved using the method of Fern and colleagues [22], by incubating nerves for 1 h at 37 °C in glucose-free aCSF (osmolarity was maintained by replacing glucose with sucrose), and switching the chamber atmosphere to 95%N2/5%CO2.
Suddenly corporate wikis are maintained by users, links to Dropbox replace e-mail attachments, and the idea of a static document is dead.Slideshare is a classic example.
This heterogeneity was maintained by extensive landscape management, which has gradually been replaced by either intensive exploitation or land abandonment.
The Walk itself is maintained by the City of LA, meaning Trump's star was replaced with taxpayer money.
The medium was replaced twice a week, and cells were maintained by serial passaging after trypsinization with 0.1% trypsin.
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