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Measures will be made in triplicate and averaged.
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Determinations were made in triplicate.
PCR analyses were made in triplicate using a TC-312 thermal cycler (Techne, Burlington, NJ, USA), in which samples underwent to a 2 min denaturing step to 94°C followed by 35 cycles of 94°C for 30 s, 54°C for 45 s, 72°C for 1 min and a final extension step at 72°C for 4 min.
Measurements were made in triplicate.
All absorbance readings were made in triplicate.
All treatments were made in triplicate.
All the measurements were made in triplicate.
Analytical determinations were made in triplicate.
All the experiments were made in triplicate.
Measurements were made in triplicate (Nasr et al. 2016).
The standard curve series were made in triplicate.
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