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With that in mind it is likely that many inferences can be made from mouse model to human immune cell biology using the bioinformatics strategy described here.
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These were made from mouse (C57/Bl6) intestine and grown for 3 days at 37°C and 5% CO2.
In the case of immunoelectron microscopy, 30-μm-thick sections were made from mouse brains fixed with a mixture of 2% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M phosphate buffer.
It was made from mouse-human antibodies that had been grown in tobacco plants.
Or to be precise, a drug called cetuximab, which is made from mice.
Co-cultures were made from mice at postnatal days 2 4.
To verify a strong DA innervation of the PFC, a subset of co-cultures were made from mice expressing GFP under the control of the TH gene promoter and the GFP signal was visualized using confocal fluorescent microscopy (Fig. 1).
For examination of tumour pathology, "gut roll" preparations were made from mice with overt signs of neoplasia as described above, fixed in 10% buffered formalin, wax embedded, and stepped serial sections stained with haematoxylin and eosin.
Further deduction of the anti-trypanosomal activity of the plant can be made from the weight gain observed in mice treated with the higher (250, 200 mg/kg) doses of extracts.
In place of oligo-dT primers in the original method, a polymerase chain reaction primer designed based on the conserved sequence of the C2H2 zinc finger protein stranded cDNA was made from various mouse tissue mRNAs, digested with FokI and BsmAI, and joined with adapters.
GXM-binding mAb 18B7 was made from a mouse immunized with a GXM-Tetanus Toxoid conjugate vaccine [8].
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