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These 'atypical' haplotypes would therefore be lost for functional and fine-mapping experiments.
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If the product of a plastid gene is essential for a plant, it can be lost from the plastome when a functional copy exists in the nuclear genome.
For example, representation of clones that express functional fusions can be lost if the fusion is expressed during amplification, and if is somewhat toxic or growth inhibitory.
Second, any biological system not required for viability may be lost, and single or low-scoring PEP-CTERM regions may be remnants of a non-functional system.
Hence the functional segment of the protein encompassing amino acids T383 and T387 which are responsible for autophosphorylation will be lost.
Conservation-based filters can enrich for functional sequences, but, as for all filters, functionally relevant sequences without sequence conservation may be lost [ 65].
Two patients were lost to functional follow-up.
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