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Two sentinel nodes in patient 4 could not be visualized using the fluorescence camera, these nodes could still be localized using the portable gamma camera and probe despite the low radioactivity count rate compared with the background.

Phosphorylation sites were localized using the Ascore[50].

At ultrastructural level Ts-PCHTP was localized using the same antibodies and protein A-gold.

After several washes in PBS, antibody complex was localized using the ABC system (Vectastain ABC Elite kit cat #PK6101, Vector Laboratories) followed by 3,3'-diaminobenzidine reaction with nickel enhancement.

These sentinel nodes were localized using the fluorescence camera.

The primary antibody was localized using the Vectastain ABC-Elite peroxidase detection system (Vector Laboratories, Burlingame, CA).

The cathepsin K derived Col II neoepitope was localized using the C2K antibody.

For tumor heterotransplants, the primary antibody was localized using the DakoCytomation ARK (Animal Research Kit Dakoo), and peroxidase (Cat. no. K3954; Dako).

The bin in which the X-chromosome inactivation center (Xic) is situated in the middle was localized using the closest gene available, that is, RLIM.

They were respectively used at 1 50, 1 100 and 1 1500 dilutions with an incubation time of 30 min. Antibody was localized using the LSAB® 2 Detection System (Dako).

The transcriptional start site of the so2426 gene was localized using the 5' RACE System for Rapid Amplification of cDNA Ends version 2.0 (Invitrogen) according to the manufacturer's instructions.