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Ci-IP3/PM can be loaded into cells non-invasively to high concentrations without activating IP3 receptors (IP3Rs).
Fluo-4 AM is a cell-permeant acetoxymethyl ester which can be loaded into cells.
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As above, the Zinpyr-1 fluorescent probe was loaded into cells by its addition to the culture medium.
A dominant-negative form of Rac, N17Rac, was loaded into cells during the wounding of monolayers to suppress the activity of cellular Rac (Figure 10A).
In brief, Rh123 (10 μM) was loaded into cells for 20 min at 37 °C.
In Fab-based live endogenous modification labeling (FabLEM), fluorescently labeled antigen-binding fragments (Fabs) are loaded into cells.
To measure mitochondrial membrane potential in cultured T cells, JC1 dye (final concentration of 2.5 μg/mL, 30 minutes at 37°C) was loaded into cells.
To measure intracellular reduced thiols, in cultured T cells, monochlorobimane (MCB) (final concentration of 40 μM, 30 minutes at 37°C) was loaded into cells.
Mt-PrsA [dialyzed into 50 mM KH2PO4 (pH 7.9), 300 mM NaCl, 1 mM DTT] was loaded into cells with two channel Epon center pieces and quartz windows.
Note also that the reduction in the rates of oxygen consumption caused by pegylation is more pronounced when the conjugates are loaded into cells than when they are in solution.
The entire EGFR model, as shown in Fig. 6, is loaded into Cell Designer and executed on a single computer.
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