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The Eckhaus equation can be linearized by making some transformations of dependent variables [28].
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Thus, this term is linearized by making the quantity U 2 in the nonlinear term a local constant such as Z m.
Furthermore, the nonlinear second-order ordinary differential equations can be linearized by a Sundman transformation.
The pUAST vector was linearized by digestion with HindIII and the ends were made blunt by Klenow fragment treatment.
The pCS2+VP16:asconstructtruct was linearized by SacII, and the capped RNA was transcribed by SP6 RNA polymerase.
The construct was linearized by NotI digestion and microinjected into an embryonic stem (ES) cell line from 129S mice.
The construct was linearized by PI- SceI and purified using Sepharose columns.
The construct was linearized by digestion with BamHI and purified for oocyte injection (Qiagen).
The construct was linearized by KpnI digestion and transformed into clone 1D4 (Fig 1C).
The ctsl1b construct [46] was linearized by NotΙ and transcribed by T7 RNA polymerase.
The myod construct [45] was linearized by XbaΙ and transcribed by T7 RNA polymerase (Roche Applied Science, Penzberg, Germany).
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