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Preparation for the retest was less intense, at least outwardly.
In particular, peak f is formed at flow rates of 400 µL/min, but is less intense at lower flow rates.
The second assumption is that herbivory and pathogen pressure is less intense at higher latitudes.
However, the MnP3 CD spectrum was significantly modified during pH 8 incubation with displacement of the main minimum from 222 to 208 nm, while the spectral changes were less intense at pH 3.
While the DAPK/pS308 signal was elevated at concentrations of 10-100 nM, but was less intense at 1 000 nM after 24 h treatment, the DAPK/pS308 signal was not detectable at 48 h and 72 h, which might be due to the increasing number of dead cells (Supplementary information, Figure S1L).
The biotic interactions (RQ) hypothesis for geographic parthenogenesis assumes, first, that herbivory and pathogen pressure is less intense at higher latitudes; and second that pressure from coevolving pests and pathogens selects for host sexual reproduction while absence of this pressure selects for asexual reproduction.
It should be noted that despite the apparently adequate doses applied (paclitaxel 175 mg m−2, cisplatin 75 mg m−2, and ifosfamide 4.5g m−2, divided over 3 days), the regimen was less intense, as it was administered at an intended 4-week interval, contrary to the usual 3-week interval pertaining to our and the other published studies so far (Zanetta et al, 1999; Choi et al, 2006).
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