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The rolling circle products were detected by hybridizing the fluorescently labeled identifier probes lin16 and lin33 (0.2 μM of each) in a mixture containing 20% formamide, 2× SSC, and 5% glycerol under coverslip for 30 min at 37°C in a humidity chamber (may be left over night at 37°C).
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The mixtures were left over night and then heated in 1.6 kilowatts microwave oven for 30 min.
Cells were left over night at 37°C in an atmosphere with 5% CO2 to differentiate into macrophages and to minimize possible gene expression changes associated with cell stress caused by the isolation procedure.
But plenty will be left over.
For workers who toiled under harsh conditions, lunchtime was a respite, with hearty portions of rice matched with whatever meat was left over from dinner the night before.
Some lumps, however, were left over.
They got what was left over.
Nothing is left over.
We feed the cat, take out the trash, wash the dishes, if any are left over from the night before.
What little food was left over from the night before came firing out, and my belly gurgled angrily as it took some small revenge in the form of heavy, wet dog farts.
Ke$ha describes her making as being "whatever's left over from the night before".[1] Obviously she doesn't put much thought into it!
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CEO of Professional Science Editing for Scientists @ prosciediting.com