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At each slice, a CCD camera records a fluorescence image of the tissue block in pixel dimensions of 10 µm × 10 µm to be later analyzed for fluorophore distribution.
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Tapes and notes from the interview are later analyzed for the evaluator's successes, misunderstandings, mistakes, and opinions.
Serum was later analyzed for levels of sodium, potassium, calcium, chloride, glucose and lactate using a Stat Profile Plus 9 blood gas instrument (Nova Biomedical Corporation, MA, USA).
The cell lysates were later analyzed for luciferase reporter expression.
Participants provided a blood sample, which was later analyzed for anti-tissue-tranglutaminase antibodies, and alongside filled in a questionnaire.
The first samples were processed without sieving at Utrecht University, where they were extracted and analyzed for endotoxin (as a marker of Gram-negative bacteria) and EPS as described previously (12), and the stored residue after extraction was later analyzed for β(1,3 -glucans (as a marker of fungal biomass) by heat extraction and inhibition enzyme immunoassay (EIA) (1,3 -glucans
However, these parameters will be later analyzed (subsection 3.4.1).
DNA prepared from whole blood samples was later analyzed by PCR for parasite species specific genotyping using established protocols [51] to confirm their infection status with the malaria parasites.
To further verify the non-infection status of the individuals in this study group, the DNA extracted from their whole blood sample was later analyzed by PCR for parasite species specific genotyping to identify those that might have asymptomatic malaria infections or to confirm their malaria infection free status using established protocols [51].
Hippocampal tissue from representative animals was later analyzed by Western blot for CREB immunoreactivity.
The ultrasound was recorded for 3 min, and the data were later analyzed.
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