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Another formulation on the basis of fluorescence experiments has been set up considering each chain to be labelled either with a Förster donor or a Förster acceptor.
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These markers were labelled either with fluorochrome FAM or HEX (Proligo, Singapore).
They were labelled either with digoxigenin-11-dUTP (344H4) or with biotin-16-dUTP (214H10, 313F4) using nick translation.
Each sample was thus hybridized multiple times while being labelled either with Cy3 or Cy5 (additional file 3, table S2).
Probes were labelled either with SpectrumGreen-dUTP (Abbott Molecular, Illinois, USA) or Cy3-dUTP (GE Healthcare UK Ltd., Little Chalfont, England) using Nick Translation Mix (Roche Applied Science, Mannheim, Germany).
The A- and D-forms of complex I in SMP were labelled either with cyanine fluorescent Cy3-NHS or Cy5-NHS esters respectively and pooled.
The A- and D-forms of complex I were labelled either with cyanine fluorescent Cy3- or Cy5-NHS esters before separation of complex I subunits by BN/double SDS-PAGE.
Virus-infected PMΦ cells were labelled either with anti-F or anti-G and examined using fluorescence scanning confocal microscopy (FSCM), which revealed a structured punctuate staining pattern on the surface of infected cells in each case.
Three micrograms of aRNA (unless otherwise specified) or 2 μg poly(A)+ RNA were labelled either with Cy5-dUTP (experimental sample) or Cy3-dUTP (reference) in a 30.4 μl reaction.
The leukocytes are labeled either with 99mTc or 111In, with 99mTc being the more commonly used radionuclide due to its nearly optimal radiation characteristics.
We used two chips in which RNAs from each sample were labeled either with cy3 or with cy5.
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