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Because our synthetic strategy allows the incorporation of any sugar headgroup and fatty acid acyl chain and involves the late-stage introduction of the label from an advanced intermediate, we expect other CD1d agonists (and potentially other bioactive glycolipids) can be labeled using this approach.
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Quartz tracer particles were labeled using the direct activation technique.
Probes were labeled using the random priming method.
Total RNA samples were labeled using the 'indirect' method [35].
Pine genomic DNA and chloroplast clones were labeled using the Megaprime DNA Labeling System (GE Healthcare).
Total RNA was labeled using the miRCURY LNA microRNA Array power labeling kit (Exiqon Inc., Denmark).
Probes were labeled using the random-primed DNA labeling kit (GE-Amersham, UK).
Approximately 2.5 µg of RNA were labeled using the Affymetrix labeling protocol (Affymetrix, Santa Clara, CA).
Diets were labeled using the following conventions.
Negative control siRNA was labeled using the kit.
cDNA was labeled using the NimbleGen Dual-Color Labeling Kit (NimbleGen) according to manufacturer's protocol.
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