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This feature of the approach is particularly useful when multiple genes need to be knocked out simultaneously, as with the Smad3-/- Smad2fl/fl Im mice that had five independently Smad3-/- Smad2fl/fl Imy modified alleleSmad3-/- Smad2fl/fl Im
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As mentioned in Section 2, reactions encoded by the same gene sets in a reduced model are knocked out simultaneously (e.g. two transketolase reactions are knocked out at once, as they both are encoded by the same tktA and tktB gene sets), which avoids cases where only a part of the reactions encoded by the same gene sets is disrupted in silico.
Amir Khan will be knocked out.
This was unexpected given the dispensability of the β subunits for viability in S. cerevisiae (which has two CK2β subunits that can be knocked out individually or simultaneously [ 14, 22, 23]) and ib Schizosaccharomyces pombe (which had a single β subunit) [ 43].
The Internet is knocked out.
Communications were knocked out.
Power was knocked out.
Both were knocked out.
"I was knocked out of bed".
Where they were knocked out.
The artist was knocked out.
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