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Continuous analysis will be kept for confirming the performance and robustness of the system.
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Finally, sepharose beads with the immunoprecipitated proteins were resuspended in 20 μl of kinase buffer (20 mM Hepes pH 7.5, 15 mM MgCl2, 5 mM EGTA, 1 mM DTT); 5 μl was kept for a western blot to confirm recovery of the HA-tagged proteins.
About 15 individuals of each line were kept for more than 10 days to confirm successful inoculation (Additional file 26: Figure S8).
DNA was denatured at 95°C for 5 min, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 45 s and extension at 72°Cfor 30 s. Final extension of 72°C was kept for 7 min. Amplicons were sequenced to confirm the genomic sequence.
Recruitment has now ended and 780 patients have been kept for final analysis, the remaining being secondary excluded mainly because sepsis was not confirmed by the adjudication committee.
He was kept for observation postoperatively for a few days and discharged after confirming patency of shunt with the help of a shunt tap and reduced ventricle size on repeat CT.
First, only SNPs confirmed by two independent PCR, three clones for each PCR, were kept for further analysis.
Mules and donkeys are kept for transportation.
The heap was kept for 2 weeks.
The mixture was kept for 5 min.
The supernatants were kept for later use.
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