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This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes.
This study has shown that SARS-CoV can be isolated in several cell lines commonly used for diagnostic and research purposes and highlights that the virus can achieve high titers in some cell lines, sometimes in the absence of CPE.
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Usutu virus (USUV) has been isolated in several African and European countries mainly from mosquitoes and birds.
Until now over 50 different suppressor (Su var)) or enhancer (E var)) mutants were isolated in several laboratories, but only a few of them have been characterized in detail.
Since then this organism has been isolated in several countries [9], and is considered one of the most important causes of ovine infertility, with a significant economic impact on the sheep industry [10].
Knockout mutations were isolated in several genes in which such alleles were not previously available.
Other pathogens were isolated in several cases such that the extent of its pathogenicity remains obscure.
WNV is transmitted mainly by mosquito vectors, although it has also been isolated in several tick species (3, 5, 13 ).
68,000 and 45,000 of 7BS and 7BL arms, respectively, corresponding to 50 ng of DNA, were isolated in several batches.
However, strains with the microsatellite profile Lmj 1, Lmj 39 and Lmj 53 were isolated in several locations at different times.
The P5CS gene was isolated in several species, such as rice (Yoshiba et al. 1995), Arabidopsis (Igarashi et al. 1997) and tomato (Fujita et al. 1998).
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