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Measurement of transcripts requires that RNA samples be isolated from tissue, for which the collection rates and cytosol volumes are difficult to estimate.
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Chondrocytes and fibroblasts were isolated from tissue and cultured in media for 2 weeks.
Wildlife was considered positive if a M. bovis strain was isolated from tissue culture.
The SVCV-C1 strain was isolated from tissue and water samples as previously described.
Total RNA was isolated from tissue punches by trizol extraction (Invitrogen, San Diego, CA).
Total RNA was isolated from tissue using the Strataprep total RNA Miniprep Kit (Stratagene) according to the manufacturer's directions.
Total RNA was isolated from tissue samples with Triazol (Invitrogen), and then cleaned with RNeasy MinElute Cleanup Kit (Qiagen).
Total RNA was isolated from tissue rat's lung homogenates and primary rat pulmonary arterial smooth muscle cells (PASMC) with Trizol® Reagent.
RNA was isolated from tissue using 1 ml of TRIzol (Invitrogen, Carlsbad, CA) and homogenization in a 5-ml glass tube.
Total RNA was isolated from tissue of mouse ventral midbrain, ventral hindbrain, and isthmus, reverse transcribed and processed for PCR [39].
Total mRNA was isolated from tissue using Totally RNA (Ambion, Austin, TX), per the manufacturer's protocol, followed by ethanol precipitation and Chromaspin column.
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