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Atrial cardiomyocytes or trabeculae can be isolated from samples of the right atrial appendage excised at the time of cardiac surgery and cardiopulmonary bypass.
However, a study in New York, NY, that tested environmental sites for AIV (H7N2) found that virus could be isolated from samples from floors, walls, and drains from the poultry areas of LBMs (8 ).
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The bacteria had been isolated from samples submitted to the University of Idaho Caine Veterinary Teaching Center as part of disease diagnostic testing.
Genomic DNA and total RNA were isolated from samples using standard techniques (Qiagen, Valencia, CA).
Total RNA was isolated from samples of human brain cortex using TRI reagent [25].
Total RNA was isolated from samples using the acid guanidinium thiocyanate-phenol-chloroform method [46].
Briefly, total RNA was isolated from samples using the Qiagen RNeasy kit (Qiagen).
Additionally, there were 10 cases where the same MLVA type was isolated from samples collected on different dates (Figure 5B); on 8 occasions, the same MLVA type was isolated from samples collected on subsequent dates at the same location.
Several bacteriophages were isolated from samples taken at a sewage plant (Jyväskylä, Finland) by the enrichment method as described below.
DNA was isolated from samples using the QIAGEN DNeasy Tissue kit (QIAGEN Inc., Valencia, CA) according to the manufacturer's instructions.
Total RNA was isolated from samples using the RNeasy mini kit (Qiagen Inc, Germantown, MD) per the manufacturer's instructions.
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