Total RNA was isolated from control and TWEAK-treated C2C12 myotubes using the Agilent total RNA isolation kit (Agilent Technologies, Palo Alto, CA).
RNA was isolated from control and forskolin-treated samples cultured for 24, 48 and 72 hours.
Total cellular RNA was isolated from control and JEV infected neurospheres at different dpi using RNAeasy Mini Kit (Qiagen, Hamburg, Germany).
Total RNA was isolated from control and hUCBSC-treated mice brains using Rneasy kit (Qiagen, Valencia, CA) and quantity were determined by A260 measurement.
Messenger RNA was isolated from control or stimulated (anti-CD3/anti-CD28 see above) lymph node cells using an mRNA capture kit (Roche) according to the manufacturer's instructions.
Total RNA was isolated from control or CHC siRNA transfected cells in a 6-well plate using the total RNA purification system (Invitrogen, Carlsbad, USA).
Exosomes were isolated from control, M. smegmatis and M. avium infected or LPS-treated (100 ng) or latex-bead fed cells using standard procedure [10], [48].
For real time polymerase chain reaction (RT-PCR) analysis and RT-PCR-based microarray analysis (RT2 Profiler PCR Array, SuperArray, Frederick, MD), total RNA was isolated from control and hUCBSC-treated cancer cells.
To confirm the improvement of mitochondrial performance observed in whole cells, we investigated oxygen consumption of mitochondria that were isolated from control and APP cells after 24 h treatment with GBE.
Total RNA was isolated from control and retinoid-stimulated cells using TRIzol and fractionated in a 1.2% agarose gel, blotted to nitrocellulose, hybridized with 32P-dCTP-labeled probes, washed and exposed to Kodak X-ray film as described [21].
