Sentence examples for be isolated for subsequent from inspiring English sources
That is, for a single notch MS3 method, the most intense ion will be isolated for subsequent MS3; for a three notch SPS method, the three most intense fragment ions will be selected and so on.
All tissues were sectioned to generate osteochondral slabs 2 to 2.5 mm thick from regions of interest for micro-computed tomography scanning and histological analysis; regions immediately adjacent were isolated for subsequent grinding and RNA isolation using a custom-made workstation as described previously [ 15].
At the end of treatment, the medium was removed and the RNA and protein were isolated for subsequent reverse transcription PCR and Western blot analysis.
At the 3- to 4-leaf stage, the seedlings showing the fastest growth rate were isolated for subsequent analysis by AFLP and cultivated to obtain progeny for subsequent phenological analysis.
Following measurement of the fluorescence signal in the relevant gated region, 5 × 10 side population cells were sorted into Eppendorf tubes on ice and centrifuged, before total RNA was isolated for subsequent mRNA expression analysis, as described below.
DNA samples of 77 breast tumors (n = 77) and 10 normal breast tissues from healthy individuals (n = 10) (Additional file 1: Table S1) and 38 ICBP breast cancer cell lines (n = 38) were isolated for subsequent DNA methylation analysis.
After 28 days, clusters of induced endothelial (iEnd) cells seemed and were isolated for further propagation and subsequent characterization.
Although this need of a high heat shock temperature resulted in complete loss of any viable yeast cells that could be cultivated again after the sort process, we were able to demonstrate that sufficient plasmid DNA can still be isolated and amplified by PCR for subsequent transformation of yeast.
