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In fact, more phosphopeptides are bound to the metal ions and more phosphopeptides can be isolated by using ammonium hydroxide as the eluent by use of glycolic acid in the loading buffer of TiO2.
The high yielding and stable strains may be isolated by using a media containing inducers of the products and devoid of repressors.
High producers can be isolated by using microscopy in conjunction with micromanipulators [ 9], or with automated cell pickers, as well as by fluorescence activated cell sorting (FACS) [ 7, 10].
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Total RNA was isolated by using an RNAqueous isolation kit (Ambion).
Total RNA was isolated by using SV total RNA isolation kit (Promega, Madison, WI) and quantified.
Small RNAs were isolated by using the mirVana miRNA isolation Kit (Ambion, Austin, TX).
Total RNA was isolated by using a TRIzol-based miRNA isolation protocol (Invitrogen).
RNA was isolated by using the Macherey Nucleospin Total RNA isolation kit.
Total RNA from cells, liver and aortic tissue was isolated by using TrizolTM reagent.
Genomic DNA was isolated by using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany).
Peptide aptamers specific for numerous proteins have been isolated by using the yeast two-hybrid system.
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