Sentence examples for be isolated by flow from inspiring English sources
Moreover, specific variants can be isolated by flow cytometry sorting and used for further analysis.
Furthermore, kidney marrow transplantation into sub-lethally irradiated animals has been developed in zebrafish (Pugach et al., 2009; Traver et al., 2004), and each major adult blood lineage can be isolated by flow cytometry (Traver et al., 2003).
First, with the characteristic of expression of specific cell surface proteins, such as cluster of differentiation 133 (CD133) and cluster of differentiation (CD44), CSCs can be isolated by flow cytometry [ 7, 8].
Subpopulations of AFCs can be isolated by flow cytometry based on the expression of specific cell marker, for example, follicle-stimulating hormone receptor (FSHR) for isolation of GCs [ 8]; however, this approach can lead to a loss of less differentiated/progenitor GCs which do not express FSHR yet.
Our strategy depended on the purification of Pax3 positive cells, which was implemented using a mouse line (Pax3 GFP/+) in which one allele of Pax3 had been targeted with a GFP coding sequence [ 6], so that cells expressing this allele could be isolated by flow cytometry.
Most commonly, single cells can be isolated by flow-cytometry, laser capture microdissection, serial dilution, using antibody-coated magnetic beads, or microfluidic-based techniques.
Until whole-genome sequencing becomes more economical, specific genomic regions can be isolated for sequencing, for example, chromosomes or derivative chromosomes can be isolated by flow-sorting, or regions of interest can be selected from the genome by sequence capture (also termed 'pull-down' or 'enrichment' Coffey et al., 2011; Hedges et al., 2011).
Fibroblast cells were infected by lentivirus particles and positive cells were isolated by flow cytometry.
LS8-Gluc-CFP and LS8-Gluc-YFP clones were isolated by flow cytometry.
On D12 16 post injection, DC subsets were isolated by flow cytometry-based sorting.
The double positive cells were isolated by flow sorting for further experiments (BD FACSAria I).
